A simple and rapid protocol of crude DNA extraction from ...

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The results of phytoplasma detection by TaqMan MGBbased realtime PCR performed on crude and purified DNA extracts showed agreement between the two methods ().Crude extraction allowed detection of phytoplasma infection in extracts from 76 out of 86 in vitro apple shoots, 29 out of 35 greenhousegrown apple plants, and 6 out of 10 greenhousegrown periwinkle plants.

Comparative study of phytochemical screening, antioxidant ...

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The aim of this work is to investigate and compare the phytochemical screening, antioxidant and antimicrobial activities of different crude extracts from dry and fresh leaves of Datura metel L. Different organic solvents including methanol, chloroform, hexane, ethyl acetate and butanol were used to prepare the crude extracts from the fresh and dry leaves.

Screening of crude extracts of six medicinal plants used ...

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Mar 11, 2005· This may go a long way in curbing administration of inappropriate concentration; a common practice among many traditional medical practitioners in Nigeria. This study represents the first preliminary report on antimethicillin resistant Staphylococcus aureus activity of the crude extracts of these medicinal plants in Lagos, Nigeria.

Development of Extraction Methods and Quantification of ...

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Extraction of safranal from cumin, to the best of our knowledge, is first time reported. The aqueous extract of cumin inhibits growth of many pathogens [15,16]. Therefore, overall purpose of this paper is to develop a better method of extraction and quantification of safranal from cumin and to study its antimicrobial activity. The crude and ...

Screening of Crude Microalgal Extracts for Antimicrobial ...

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The biofilm chemical and physical properties restrict access of chemotherapeutic agents. Therefore, there is a need to enhance the antimicrobial effects of current chemotherapeutic agents. Microalgae live in competitive environments that include filmforming, opportunistic pathogens S. maltophilia and C. albicans. Extracts from 5 diverse ...

Valorification of crude glycerol for pure fractions of ...

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Testing the effect of these parameters was determined: biomass, DHA and βcarotene concentration. The highest production by S. limacinum was obtained at 25 °C, while using a quantity of 90 g/L of glycerol (crude or pure) as a carbon source. Temperature was the main factor that influenced the biosynthesis of …

Extraction of Crude Metal from Concentrated Ore (In Hindi ...

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Extraction of Crude Metal from Concentrated Ore What is Crude Metal? ... Concentration of Ore Isolation of metal from the concentrated Ore Purification of the metal Isolation of metal from the concentrated Ore involves the refining of metals from the compounds. ... The sulphur in the ore, at high temperature reacts with the oxygen to form ...

Cleaning Excavated Soil Using Extraction Agents A Stateof ...

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EPA/60G/2C9/034 June 1989 CLEANING EXCAVATED SOIL USING EXTRACTION AGENTS: A STATEOFTHEART REVIEW by R. Raghavan E. Coles D. D1etz Foster Wheeler Enviresponse, Inc. Livingston, NJ 07039 Contract Number 68033255 Project Officer Darlene Williams Releases Control Branch Superfund Technology Demonstration Division Risk Reduction Engineering Laboratory …

Evaluating antibacterial and anticancer activity of crude ...

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Extraction of crude extracts from bacterial endophytes ... broth was added to bring the volume of the dissolved crude extract to a concentration of 32 mg/ml as the stock solution. ... This may suggest that extracts from P. moraviensis could be used as antibacterial agents. Rahnella aquatilis crude extract had MIC values ranging between 0 ...

Protocol for Isolation of Genomic Dna from Dry and Fresh ...

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valuable pharmacokinetic properties of medicinal plants agentmercaptoethanol in addition to proteinase K which interfere with the isolation process, tend to co which removes protein [9]. Others like Reichardt and purify with DNA and interact irreversibly with proteins Rogers (1993) have used high CTAB concentration which and nucleic acids [7].

OPTIMIZATION OF DEMULSIFIER FORMULATION FOR …

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OPTIMIZATION OF DEMULSIFIER FORMULATION FOR SEPARATION OF WATER ... agents in the crude oil. Table 1: Chemical characteristics of crude oils. Crude oils ... Extraction of Asphaltene Crude oil was dispersed in npentane or nheptane at a concentration of 1:5 (v/v). The mixture was then

Natural Products Chemistry Research

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obtained crude extract was concentrated to semisolid brown coloured mass. Extraction of crude curcuminoids from oleo resin One gram of crude extract was added to 25 mL of Hexane and kept aside for 12 hrs. After 12 hrs, the solution with lump of crude extract was stirred using magnetic stirrer at 600 rpm for 3 hrs. During the

Mercury in extraction and refining process of crude oil ...

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Mercury in extraction and refining process of crude oil and natural gas Author: Gerard Subirachs Sa nchez Supervisor: Dr. Euan Bain This thesis was submitted as part of the requirement for the MEng. Degree in Engineering School of Engineering, Univ. of Aberdeen 20May2013

Low viscosity, high concentration drag reducing agent and ...

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Mar 31, 1998· Low viscosity, high concentration drag reducing agents may be prepared by slowly adding a liquid, nonsolvent ( isopropyl alcohol) for a drag reducing polymer ( a polyalphaolefin) to a mixture of the polymer and the solvent ( kerosene) in which the polymer is dissolved.

METHANOL EXTRACTION METHOD Google Docs

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Methanol, also known as methyl alcohol, wood alcohol, wood naphtha or wood spirits, is a chemical with formula C H 3 O H (often abbreviated MeOH). It is the simplest alcohol, and is a light, volatile, colorless, flammable, liquid with a distinctive odor that is very similar to but slightly sweeter than ethanol (drinking alcohol). At room temperature it is a polar liquid and is used as an ...

Grinding And Extraction Protocols Products From Plants

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Apr 29, 2019· General Extraction Protocols for Biologically Important Organic Compounds. The primary ways for extraction of organic molecules of interest to biologists and medical investigators involve breaking open the cells of the organism under investigation. Cell rupturing is accomplished in a …

Drugs from Plants : Extraction, Purification Analysis.

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Feb 24, 2018· Drugs from Plants : Extraction, Purification Analysis. 1. Dr. A. Amsavel 2. Content Source Of Drugs History Of Medicines Used From Plants Recent Development Technology Pharmacology Of Photochemical Plants, Medicinal Chemistry Treatments Type Of Extractions Isolation And Purification Analysis of Drug Job Opportunities In Pharma Industries References

(PDF) EXTRACTION, STABILITY AND SEPARATION OF …

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An attempt was made to separate color components of the extract by developing HPTLC method. Results: The yield of microwave oven extraction of colorant is found to be % under the optimized conditions set at the temperature of 70˚C, 50 minutes time, % HCl concentration in solvent and 1:60 ratio of sample to solvent.

Caffeine Extraction Lab Report2

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into the aqueous layer. Sodium carbonate is added to the extraction medium to ensure that the acidic components in the tea leaves remain water soluble and that caffeine is the free base. Sodium carbonate is basic. Tannins are acidic compounds with a high molecular weight that have an –OH directly bound to an aromatic ring.

Nature’s recyclers: anaerobic microbial communities drive ...

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Introduction. The conversion of organic waste material into sustainable, valueadded products is a pressing global need. In nature, the degradation and recycling of organic carbon are largely mediated by anaerobic microorganisms that work together to divideandconquer the difficult biocatalytic steps of hydrolysis [1, 2, 3, 4].Industry has benefited from a number of such anaerobic strains ...

Making Cannabis Distillate | Precision Blog

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Cannabis distillation is a postprocessing extraction technique done subsequent to an initial solventbased extraction. In practice, and by way of example, the raw oil (sometimes referred to as “crude” oil) from the cannabis plant will first be extracted via the use of a PX1 or PXP system.

Overview of Enhanced Oil Recovery Ultimate EOR

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Injecting a high pH agent (NaOH, Na 2 CO 3, etc.) dissolved in water What: How: Agent reacts with component (s) in acidic (active) crude oil to form surfactant. More effective in crude oils with higher acidity (usually below 20° API) and in sandstone reservoirs below 200°F Alteration of wettability

Extraction techniques of medicinal plants

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Extraction techniques of Medicinal plants Extraction, as the term is used pharmaceutically, involves the separation of medicinally active portions of plant or animal tissues from the inactive or inert components by using selective solvents in standard extraction procedures. The products so obtained from plants are relatively

Optimization of Processing Parameters for Extraction of ...

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Jun 23, 2014· It should be noted that the extracted amylase enzyme from dragon peel showed specific characteristics such as thermostability at high temperature, high activity at acidic pH, and high stability of the enzyme in presence of ionic and nonionic surfactant agents. The optimisation of the extraction process should result in achieving the maximum ...

Concept of standardization, extraction and

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c. Soxhlet extraction: Soxhlet extraction is only required where the desired compound has a limited solubility in a solvent, and the impurity is insoluble in that solvent. If the desired compound has a high solubility in a solvent then a simple filtration can be used to …

Antibacterial Activities of Crude extracts of Some East ...

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high polarity of methanol which is effective for more consistent extraction of different types of sesquiterpenoids particularly furanosesquiterpenoids, diterpenes, triterpenes and sterols [10]. It has been reported that crude extracts and essential oils from medicinal plants exercise antimicrobial

Can someone list out the methods of extraction (crude ...

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Can someone list out the methods of extraction (crude extract), compounds isolation, purification, identification and structural elucidation? ... concentration of crude extract to contain certain ...

ANTIMICROBIAL ACTIVITY OF CRUDE EXTRACTS OF SOME …

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ANTIMICROBIAL ACTIVITY OF CRUDE EXTRACTS OF SOME FOLK MEDICINAL PLANTS AGAINST BACTERIA CAUSING GASTROINTESTINAL INFECTIONS Dr Maya Verma Department of Botany, University of Rajasthan, Jaipur ABSTRACT: The present study was carried out to evaluate the antimicrobial activity of methanol, petroleum and

Extraction techniques of medicinal plants

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Extraction techniques of Medicinal plants Extraction, as the term is used pharmaceutically, involves the separation of medicinally active portions of plant or animal tissues from the inactive or inert components by using selective solvents in standard extraction procedures. The products so …

Concentration and characterization of microalgae proteins ...

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Many methods are available for the concentration of proteins; however, most are not easily scalable due to costs, the need of specialized instruments and skilled workers or are very timeconsuming. Threephase partitioning (TPP) is a separation technique that has gained a lot of interest due to its rapid, simple and scalable use for concentration, isolation and decontamination of proteins from ...

Antimicrobial screening from crude plant extract

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Antimicrobial screening from crude plant extract I want to evaluate the antimicrobial activity of my plant extract. But I don't know how to prepare the extract solution.

Crude Metal From Concentrated Ore | What Is Extraction Of ...

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Isolation of metal from the concentrated Ore involves the refining of metals from the compounds it is found with to its original state. The concentrated ore is first converted to an oxide after which metal extraction is done by reducing it from an oxide to metal using a suitable reducing agent.

(PDF) Extraction of Solasodine from Dry Fruits and Leaves ...

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The optimum concentration of 2propanol for the extraction of crude glycosides was 70%. The suitable hydrolysis condition of solasodine from crude glycosides was by 1 N hydrochloric acid in 2propanol. Pure solasodine from both fruits and leaves of Solanum laciniatum Ait. was obtained without any requirement of column chromatography.

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